ISG15 Protein (AA 1-157) (Calmodulin (CaM))

Catalog No. ABIN666864

This Recombinant ISG15 protein is expressed in Escherichia coli (E. coli).

Quick Overview for ISG15 Protein (AA 1-157) (Calmodulin (CaM)) (ABIN666864)

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 98 %

Product Details

Protein Characteristics: AA 1-157

Purification tag / Conjugate: This ISG15 protein is labelled with Calmodulin (CaM).

Sequence: MGWDLTVKM LAGNEFQVSL SSSMSVSELK AQITQKIGVH AFQQRLAVHP SGVALQDRVP LASQGLGPGS TVLLVVDKCD EPLNILVRNN KGRSSTYEVR LTQTVAHLKQ QVSGLEGVQD DLFWLTFEGK PLEDQLPLGE YGLKPLSTVF MNLRLRGG

Characteristics: ISG15, 1-157 aa, Human, Calmodulin tagged, Recombinant, E.coli

Purification: > 98% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 1 mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Alternative Name: ISG15

Background: The interferon-stimulated gene, 15 kDa (ISG15) is an interferon regulated gene that is induced as a primary response to diverse microbial and cell stress stimuli, and encodes the founding member of the ubiquitin-like protein family. ISG15 shares several common properties with other ubiquitin-like molecules (uBLs), but its activity is tightly regulated by specific signaling pathways that have a role in innate immunity. Recombinant human ISG15 was expressed in E. coli and purified by conventional chromatography techniques.

Molecular Weight: 34 kDa (308aa) confirmed by MALDI-TOF

NCBI Accession: NP_005092