ISG15 Protein (His tag)

Catalog No. ABIN7319576

Product Details

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ISG15 protein is labelled with His tag.

Purpose: Recombinant Human ISG15/G1P2 Protein (His Tag)

Sequence: Gly2-Gly157(Ser83Asn)

Characteristics: Recombinant Human ISG15 is produced by our E.coli expression system and the target gene encoding Gly2-Gly157(Ser83Asn) is expressed with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 100 mM NaCl, pH 8.0.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Alternative Name: ISG15/G1P2 (ISG15 Products)

Synonyms: G1P2 Protein, IFI15 Protein, IP17 Protein, UCRP Protein, hUCRP Protein, ISG15 Protein, g1p2 Protein, si:rp71-1c23.2 Protein, G1p2 Protein, IGI15 Protein, Irfp Protein, ISG17 Protein, ISG17/UCRP Protein, ISG15 ubiquitin-like modifier Protein, ISG15 Protein, isg15 Protein, Isg15 Protein

Background:

Background: Ubiquitin-Like Protein ISG15 (ISG15) is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha and -beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. ISG15 becomes conjugated to a diverse set of proteins after IFN-alpha/beta stimulation or microbial challenge. The functions or biochemical consequences ISG15 conjugation to proteins are not yet known, but it appears that this modification does not target proteins for proteasomal degradation. ISG15 shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. Upon interferon treatment, ISG15 can be detected in both free and conjugated forms, and is secreted from monocytes and lymphocytes where it can function as a cytokine. In the cell, ISG15 co-localizes with intermediate filaments and ISGylation may modulate the JAK-STAT pathway or certain aspects of neurological disease.

Synonym: Ubiquitin-Like Protein ISG15, Interferon-Induced 15 kDa Protein, Interferon-Induced 17 kDa Protein, IP17, Ubiquitin Cross-Reactive Protein, hUCRP, ISG15, G1P2, UCRP

Molecular Weight: 18.2 kDa

UniProt: P05161