Aldo-Keto Reductase 1B10/AKR1B10 (AA 1-316) (Active) Protein

Catalog No. ABIN667103

This Recombinant Aldo-Keto Reductase 1B10/AKR1B10 protein is produced in Escherichia coli (E. coli).

Quick Overview for Aldo-Keto Reductase 1B10/AKR1B10 (AA 1-316) (Active) Protein (ABIN667103)

Target: Aldo-Keto Reductase 1B10/AKR1B10

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Enzyme Activity Assay (EAA), SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-316

Sequence: MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY

Characteristics: AKR1B10, 1-316 aa, Human, Recombinant, E.coli

Purification: > 95% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 1,500pmol/min/ug, and was obtained by measuring the decrease of NADP in absorbance at 340nm resulting from NADPH at pH 7.0 at 37C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Aldo-Keto Reductase 1B10/AKR1B10

Background: AKR1B10, also known as Aldo-keto reductase family 1, member B10, AKR1B10 is a monomeric protein that efficiently catalyzes the reduction of aromatic and aliphatic aldehydes and ketones. AKR1B10 is ubiquitously expressed in many human tissues but is highly expressed in small intestine, colon and adrenal gland. This protein is pathogenically involved in diabetic complications and has been reported that AKR1B10 is overexpressed in human tumors, such as liver, breast, and lung cancer, and may play a critical role in the development and progression of cancer. Recombinant human AKR1B10 protein was expressed in E. coli and purified by using conventional chromatography.

Molecular Weight: 36 kDa (316aa) confirmed by MALDI-TOF

NCBI Accession: NP_064695