SAE1 Protein (AA 1-346) (His-T7)
Quick Overview for SAE1 Protein (AA 1-346) (His-T7) (ABIN667139)
Target
See all SAE1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-346
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Purification tag / Conjugate
- This SAE1 protein is labelled with His-T7.
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Sequence
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MVEKEEAG GGISEEEAAQ YDRQIRLWGL EAQKRLRASR VLLVGLKGLG AEIAKNLILA GVKGLTMLDH EQVTPEDPGA QFLIRTGSVG RNRAEASLER AQNLNPMVDV KVDTEDIEKK PESFFTQFDA VCLTCCSRDV IVKVDQICHK NSIKFFTGDV FGYHGYTFAN LGEHEFVEEK TKVAKVSQGV EDGPDTKRAK LDSSETTMVK KKVVFCPVKE ALEVDWSSEK AKAALKRTTS DYFLLQVLLK FRTDKGRDPS SDTYEEDSEL LLQIRNDVLD SLGISPDLLP EDFVRYCFSE MAPVCAVVGG ILAQEIVKAL SQRDPPHNNF FFFDGMKGNG IVECLGPK -
Characteristics
- SAE1, 1-346aa, Human, His-T7 tag, E.coli
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SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-350) protein (His tag)
custom-made SAE1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) protein (His tag)custom-made SAE1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) protein (His tag)SAE1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) protein (GST tag)SAE1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
SUMO1 Activating Enzyme Subunit 1 (SAE1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)SAE1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
SUMO1 Activating Enzyme Subunit 1 (SAE1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SAE1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
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Alternative Name
- SUMO E1/SAE1
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Background
- SAE1, also known as AOS1, belongs to the ubiquitin-activating E1 family of proteins and plays an important role in the first step of the uBL1 conjugation pathway. Proteins conjugated to ub are marked for progressive degradation by the 26S Proteasome. SAE1, which is a dimeric enzyme, functions as a uBLI E1 ligase mediating the ATP-dependent activation of uBL1. This protein can bind with uBLE1A and uBLE1B to form a heterodimer which can bind uBL1. Recombinant human SAE1 protein, fused to His-T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 42.2 kDa (378aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_005491
Target
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