UGDH Protein (AA 1-493) (His tag)

Catalog No. ABIN5855069

Product Details

Target: UGDH (UDP-Glucose 6-Dehydrogenase (UGDH))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-493

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UGDH protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVEIKKI CCIGAGYVGG PTCSVIAHMC PEIRVTVVDV NEARINAWNS PTLPIYEPGL KEVVESCRGK NLFFSTNIDD AIREADLVFI SVNTPTKTYG MGKGRAADLK YIEACARRIV QNSNGYKIVT EKSTVPVRAA ESIRRIFDAN TKPNLNLQVL SNPEFLAEGT AIKDLKNPDR VLIGGDETPE GQKAVRALCA VYEHWVPKEK ILTTNTWSSE LSKLAANAFL AQRISSINSI SALCEATGAD VEEVATAIGM DQRIGNKFLK ASVGFGGSCF QKDVLNLVYL CEALNLPEVA RYWQQVIDMN DYQRRRFASR IIDSLFNTVT DKKIAILGFA FKKDTGDTRE SSSIYISKYL MDEGAHLHIY DPKVPREQIV VDLSHPGVSA DDQVSRLVTI SKDPYEACDG AHALVICTEW DMFKELDYER IHKKMLKPAF IFDGRRVLDG LHSELQTIGF QIETIGKKVS SKRIPYTPGE IPKFSLQDPP NKKPKV

Purity: > 90 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of UDP-glucose to UDP-glucuronate per minute at pH 8.7 at 37C

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM MES buffer (  pH 5.0) containing 20 % glycerol, 150  mM NaCl, 1  mM EDTA

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: UGDH (UDP-Glucose 6-Dehydrogenase (UGDH))

Alternative Name: Ugdh (UGDH Products)

Synonyms: 8310 Protein, BG01214 Protein, CG10072 Protein, Dmel\\CG10072 Protein, Sgl Protein, UDP-GlcDH Protein, UDPGDH Protein, XIII Protein, kiwi Protein, l(3)05007 Protein, l(3)08310 Protein, l(3)10503 Protein, l(3)65CEd Protein, l(3)SG9 Protein, ska Protein, sl Protein, ik:tdsubc_2a8 Protein, tdsubc_2a8 Protein, xx:tdsubc_2a8 Protein, gdh Protein, ugd Protein, ugdh Protein, ugdhb Protein, xugdh Protein, udpgdh Protein, udpg Protein, ugdha Protein, BA5434 Protein, SCBAC19G2.07 Protein, AGR_L_1413 Protein, udg Protein, GDH Protein, UGD Protein, Udpgdh Protein, sugarless Protein, UDP-glucose 6-dehydrogenase Protein, UDP-glucose 6-dehydrogenase L homeolog Protein, UDP-glucose 6-dehydrogenase S homeolog Protein, UDP-glucose dehydrogenase WcvB Protein, UDP-glucose dehydrogenase Protein, sgl Protein, ugdh Protein, ugdh.L Protein, UGDH Protein, ugdh.S Protein, hasB Protein, ugdH Protein, ugd Protein, SCO3052 Protein, wcvB Protein, CNL06460 Protein, Ugdh Protein

Background: Ugdh, also known as UDP-glucose 6-dehydrohenase, is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. Recombinant mouse Ugdh, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 57.2 kDa (516aa) confirmed by MALDI-TOF

NCBI Accession: NP_033492

UniProt: O70475

Pathways: Glycosaminoglycan Metabolic Process