Luciferase Protein (AA 1-550) (His tag)

Catalog No. ABIN667203

This Recombinant Luciferase protein is expressed in Escherichia coli (E. coli).

Quick Overview for Luciferase Protein (AA 1-550) (His tag) (ABIN667203)

Target: Luciferase

Protein Type: Recombinant

Origin: Firefly

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 by SDS - PAGE

Product Details

Protein Characteristics: AA 1-550

Purification tag / Conjugate: This Luciferase protein is labelled with His tag.

Characteristics: Luciferase, 1-550aa, Firefly, His tag, E.coli

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Storage: 4 °C

Target Details

Target: Luciferase

Background: Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. Recombinant firefly Luciferase, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Luciferin 4-monooxygenase, LUC. NCBI no.: BAF48390

Molecular Weight: 62.9 kDa (571aa), confirmed by MALDI-TOF