Luciferase Protein (AA 1-311) (His tag)
Origin: Sea Pansy
Host: Escherichia coli (E. coli)
Recombinant
> 95 % by SDS-PAGE
SDS
Active
1 image
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- Target See all Luciferase Proteins
- Luciferase
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-311
- Origin
- Sea Pansy
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Luciferase protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSHMTSKVY DPEQRKRMIT GPQWWARCKQ MNVLDSFINY YDSEKHAENA VIFLHGNAAS SYLWRHVVPH IEPVARCIIP DLIGMGKSGK SGNGSYRLLD HYKYLTAWFE LLNLPKKIIF VGHDWGACLA FHYSYEHQDK IKAIVHAESV VDVIESWDEW PDIEEDIALI KSEEGEKMVL ENNFFVETML PSKIMRKLEP EEFAAYLEPF KEKGEVRRPT LSWPREIPLV KGGKPDVVQI VRNYNAYLRA SDDLPKMFIE SDPGFFSNAI VEGAKKFPNT EFVKVKGLHF SQEDAPDEMG KYIKSFVERV LKNEQ
- Purity
- > 95 % by SDS-PAGE
- Biological Activity Comment
- Specific activity is >1X109 light units/mg. One luciferase enzyme units will produce one Relative Light Unit (RLU) at pH7.5 at 25C.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. 20 mM Tris-HCl ( pH 8.0) containing 1 mM DTT, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- Luciferase
- Abstract
- Luciferase Products
- Synonyms
- hypothetical protein Protein, luciferase Protein, C25G6.3 Protein, Gbro_2186 Protein
- Background
- LUC, also known as luciferase. LUC catalyzes the oxidative decarboxylation of coelenterazine in the presence of dissolvedoxygen to yield oxyluciferin, CO2, and blue light. In vivo, the excited state luciferin-luciferase complex undergoes the process of noradiative energy transfer to an accessory protein, green fluorescent protein, which results in green bioluminescence. In vitro, it emits blue light in the absence of any green fluorescent protein. Recombinant renilla reniformis LUC, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 38.5 kDa (335aa) Confirmed by MALDI-TOF
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