LIF Protein (AA 23-202) (His-GST)
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- Target See all LIF Proteins
- LIF (Leukemia Inhibitory Factor (LIF))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-202
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This LIF protein is labelled with His-GST.
- Application
- SDS-PAGE (SDS)
- Sequence
- MHHHHHHMSP ILGYWKIKGL VQPTRLLLEY LEEKYEEHLY ERDEGDKWRN KKFELGLEFP NLPYYIDGDV KLTQSMAIIR YIADKHNMLG GCPKERAEIS MLEGAVLDIR YGVSRIAYSK DFETLKVDFL SKLPEMLKMF EDRLCHKTYL NGDHVTHPDF MLYDALDVVL YMDPMCLDAF PKLVCFKKRI EAIPQIDKYL KSSKYIAWPL QGWQATFGGG DHPPKSDLVP RGSHMSPLPI TPVNATCAIR HPCHNNLMNQ IRSQLAQLNG SANALFILYY TAQGEPFPNN LDKLCGPNVT DFPPFHANGT EKAKLVELYR IVVYLGTSLG NITRDQKILN PSALSLHSKL NATADILRGL LSNVLCRLCS KYHVGHVDVT YGPDTSGKDV FQKKKLGCQL LGKYKQIIAV LAQAF
- Characteristics
- LIF, 23-202aa, Human, His-GST tag, E.coli
- Purity
- > 90% by SDS-PAGE
- Top Product
- Discover our top product LIF Protein
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Leukemia Inhibitory Factor (LIF) (AA 23-202) protein
LIF Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) proteinLIF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Leukemia Inhibitory Factor (LIF) (AA 25-203) protein (Fc Tag)LIF Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (Active) proteinLIF Origin: Rat Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (GST tag)LIF Origin: Human, Mouse Host: Escherichia coli (E. coli) Recombinant >95 % (SDS-PAGE) SDS Active
Leukemia Inhibitory Factor (LIF) (AA 24-203) protein (His tag)LIF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (His tag)LIF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Leukemia Inhibitory Factor (LIF) (AA 1-202) protein (GST tag)LIF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Leukemia Inhibitory Factor (LIF) proteinLIF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (His tag)LIF Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS, AcA Active
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 50mM NaCl
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- LIF (Leukemia Inhibitory Factor (LIF))
- Alternative Name
- LIF (LIF Products)
- Background
- Leukemia inhibitory factor, also known as LIF, is a pleiotropic cytokine that is expressed by a wide variety of cells including activated T lymphocytes, monocytes, mast cells and neuronal cells. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Recombinant human LIF protein, fused to His GST-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 47.2 kDa (415aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_002300
- Pathways
- JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
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