LIF Protein (AA 25-203) (Fc Tag)
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- Target See all LIF Proteins
- LIF (Leukemia Inhibitory Factor (LIF))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 25-203
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This LIF protein is labelled with Fc Tag.
- Purpose
- Mouse LIF Protein
- Sequence
- Pro25-Phe203
- Characteristics
- Recombinant Mouse LIF Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Pro25-Phe203.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Top Product
- Discover our top product LIF Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- LIF (Leukemia Inhibitory Factor (LIF))
- Alternative Name
- LIF (LIF Products)
- Synonyms
- CDF Protein, DIA Protein, HILDA Protein, MLPLI Protein, LIF, interleukin 6 family cytokine Protein, leukemia inhibitory factor Protein, LIF Protein, Lif Protein
- Background
- Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.
- Molecular Weight
- 46.5 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on Tris-Bis PAGE result.
- UniProt
- P09056
- Pathways
- JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
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