Ecotin (AA 21-162) protein (His tag)
-
- Target
- Ecotin
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-162
-
Origin
- E. coli
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- SDS-PAGE (SDS)
- Characteristics
- Ecotin, 21-162aa, E.coli, His tag, E.coli
- Purity
- > 95 % by SDS - PAGE
-
-
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 50mM NaCl, 1mM DTT, 10% glycerol
- Storage
- 4 °C
-
- Target
- Ecotin
- Synonyms
- 2 Protein, EAR-13 Protein, EAR-2 Protein, ECP Protein, ECP 2 Protein, Rnase2 Protein, EAR Protein, R1 Protein, R7 Protein, Rnase3 Protein, RNS3 Protein, eosinophil-associated, ribonuclease A family, member 2 Protein, eosinophil-associated, ribonuclease A family, member 11 Protein, ribonuclease A family member 3 Protein, Ear2 Protein, Ear11 Protein, RNASE3 Protein
- Background
- Ecotin (E. coli serine protease inhibitor) inhibits pancreatic serine proteases. This protein inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases. The strength of inhibition does not appear to be correlated with a particular protease specificity. Recombinant E. coli Ecotin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: ECK2201, eti, JW2197. NCBI no.: NP_416713
- Molecular Weight
- 18.3 kDa (163aa), confirmed by MALDI-TOF
-