ATF3 Protein (AA 1-181)
Quick Overview for ATF3 Protein (AA 1-181) (ABIN667497)
Target
See all ATF3 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-181
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Sequence
- MMLQHPGQVS ASEVSASAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ NKHLCHRMSS ALESVTVSDR PLGVSITKAE VAPEEDERKK RRRERNKIAA AKCRNKKKEK TECLQKESEK LESVNAELKA QIEELKNEKQ HLIYMLNLHR PTCIVRAQNG RTPEDERNLF IQQIKEGTLQ S
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Characteristics
- ATF3, 1-181aa, Human, E.coli
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Purification
- > 90% by SDS-PAGE
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Activating Transcription Factor 3 (ATF3) (AA 1-181) protein (His tag)
custom-made ATF3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Activating Transcription Factor 3 (ATF3) (AA 1-181) protein (GST tag)ATF3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Activating Transcription Factor 3 (ATF3) (AA 1-181) proteinATF3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
Activating Transcription Factor 3 (ATF3) protein (GST tag,His tag)ATF3 Origin: Human Host: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Activating Transcription Factor 3 (ATF3) protein (His tag)ATF3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 2M urea, 20 % glycerol
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- ATF3 (Activating Transcription Factor 3 (ATF3))
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Alternative Name
- ATF3
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Background
- ATF3 (activating transcription factor 3) is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription, it is presumed, by sequestering inhibitory co-factors away from the promoter.
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Molecular Weight
- 20.6 kDa (181aa)
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NCBI Accession
- NP_001665
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Pathways
- Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response
Target
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