Ppa (AA 1-176) protein (His tag)

Catalog No. ABIN667500

Product Details

Target: Ppa

Protein Type: Recombinant

Protein Characteristics: AA 1-176

Origin: E. coli

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: His tag

Application: SDS-PAGE (SDS)

Characteristics: ppa, 1-176aa, E.coli, His tag, E.coli

Purity: > 95 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl

Storage: 4 °C

Target Details

Target: Ppa

Background: Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations. Recombinant E.coli ppa protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: inorganic pyrophosphatase, ECK4222, JW4185. NCBI no.: NP_418647

Molecular Weight: 21.9kDa (196aa), confirmed by MALDI-TOF