SGK1 Protein (AA 60-431) (His tag)
Quick Overview for SGK1 Protein (AA 60-431) (His tag) (ABIN667573)
Target
See all SGK1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 60-431
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Purification tag / Conjugate
- This SGK1 protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MISQPQEPEL MNANPSPPPS PSQQINLGPS SNPHAKPSDF HFLKVIGKGS FGKVLLARHK AEEVFYAVKV LQKKAILKKK EEKHIMSERN VLLKNVKHPF LVGLHFSFQT ADKLYFVLDY INGGELFYHL QRERCFLEPR ARFYAAEIAS ALGYLHSLNI VYRDLKPENI LLDSQGHIVL TDFGLCKENI EHNSTTSTFC GTPEYLAPEV LHKQPYDRTV DWWCLGAVLY EMLYGLPPFY SRNTAEMYDN ILNKPLQLKP NITNSARHLL EGLLQKDRTK RLGAKDDFME IKSHVFFSLI NWDDLINKKI TPPFNPNVSG PNDLRHFDPE FTEEPVPNSI GKSPDSVLVT ASVKEAAEAF LGFSYAPPTD SFL
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Characteristics
- SGK1, 60-431 aa, Human, His tag, HI-5
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serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-431) protein (His tag)
custom-made SGK1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-431) protein (His tag)custom-made SGK1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-431) protein (GST tag)SGK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
serum/glucocorticoid Regulated Kinase 1 (SGK1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SGK1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- Liquid. 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 0.2M NaCl,2mM DTT,0.1mM PMSF
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))
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Alternative Name
- SGK1
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Background
- SGK1, also known as serum-and glucocorticoid-regulated kinase, is a serine/threonine protein kinase and a member of the AGC subfamily, which includes protein kinases A, G, and C. This protein plays an important role in activating certain potassium, sodium and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. It is activated in vitroby 3-phosphoinositide-dependent protein kinase-1 (PDK-1) and in vivoin response to signals that activate phosphatidylinositol (PI) 3-kinase. Recombinant human SGK1 protein was expressed with N-terminal His-tag in High-Five cells using baculovirus expression system and purified by using conventional chromatography techniques.
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Molecular Weight
- 44.5 kDa (393aa)
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NCBI Accession
- NP_005618
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Pathways
- MAPK Signaling, Notch Signaling, Steroid Hormone Mediated Signaling Pathway
Target
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