ABHD14B Protein (AA 1-210) (His tag)

Catalog No. ABIN667663

Recombinant ABHD14B protein expressed in Escherichia coli (E. coli).

Quick Overview for ABHD14B Protein (AA 1-210) (His tag) (ABIN667663)

Target: ABHD14B (Abhydrolase Domain Containing 14B (ABHD14B))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-210

Purification tag / Conjugate: This ABHD14B protein is labelled with His tag.

Sequence: MAASVE QREGTIQVQG QALFFREALP GSGQARFSVL LLHGIRFSSE TWQNLGTLHR LAQAGYRAVA IDLPGLGHSK EAAAPAPIGE LAPGSFLAAV VDALELGPPV VISPSLSGMY SLPFLTAPGS QLPGFVPVAP ICTDKINAAN YASVKTPALI VYGDQDPMGQ TSFEHLKQLP NHRVLIMKGA GHPCYLDKPE EWHTGLLDFL QGLQ

Characteristics: ABHD14B, 1-210aa, Human, His tag, E.coli

Purification: > 95% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol, 100 mM NaCl

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: ABHD14B (Abhydrolase Domain Containing 14B (ABHD14B))

Alternative Name: ABHD14B

Background: ABHD14B (abhydrolase domain containing 14B) belongs to the AB hydrolase superfamily. This protein contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing (ABHD) gene subfamily is comprised of 15 mostly uncharacterized members. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro). It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 25kDa (234aa) confirmed by MALDI-TOF

NCBI Accession: NP_116139