ABHD14B Protein (AA 1-210) (His tag)

Catalog No. ABIN7559828

This Recombinant ABHD14B protein is expressed in HEK-293 Cells.

Quick Overview for ABHD14B Protein (AA 1-210) (His tag) (ABIN7559828)

Target: ABHD14B (Abhydrolase Domain Containing 14B (ABHD14B))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-210

Purification tag / Conjugate: This ABHD14B protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Abhd14b Protein expressed in mammalian cells.

Sequence: MAGVDQHEGT IQVQGQNLFF RETRPGSGQP VRFSVLLLHG IRFSSETWQN LGTLQRLAEA GYRAVAIDLP GLGRSKEAAA PAPIGEPAPG SFLAAVVDTL ELGPPVVISP SLSGMYSLPF LVAPGSQLRG FVPVAPICTD KINAVDYASV KTPALIVYGD QDPMGSSSFQ HLKQLPNHRV LVMEGAGHPC YLDKPDEWHK GLLDFLQGLA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ABHD14B (Abhydrolase Domain Containing 14B (ABHD14B))

Alternative Name: Abhd14b

Background: Putative protein-lysine deacylase ABHD14B (EC 2.3.1.-) (Alpha/beta hydrolase domain-containing protein 14B) (Abhydrolase domain-containing protein 14B),FUNCTION: Acts as an atypical protein-lysine deacetylase in vitro. Catalyzes the deacetylation of lysine residues using CoA as substrate, generating acetyl-CoA and the free amine of protein-lysine residues. Additional experiments are however required to confirm the protein-lysine deacetylase activity in vivo. Has hydrolase activity towards various surrogate p-nitrophenyl (pNp) substrates, such as pNp-butyrate, pNp-acetate and pNp-octanoate in vitro, with a strong preference for pNp-acetate. May activate transcription. {ECO:0000250|UniProtKB:Q96IU4}.

Molecular Weight: 22.5 kDa

UniProt: Q8VCR7

AlphaFold: Q8VCR7