Annexin V Protein (His tag)

Catalog No. ABIN6699527

Supplier Product No.: 009-001-r40s

Product Details

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Annexin V protein is labelled with His tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-r40s

Supplier: Rockland

Purpose: Annexin 5 recombinant protein-HIS Epitope

Purification: Recombinant full-length human Annexin 5 Protein was expressed by E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.

Purity: >95%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Application_Note: Annexin 5 Protein is suitable for use in Western Blot. Expect a band approximately ~ 37 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: Annexin 5 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 2 mM DTT, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Alternative Name: ANXA5 (ANXA5 Products)

Background:

Synonyms: ANXA5, PP4, ANX5, ENX2, Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, CBP-I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, PAP-I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, VAC-alpha

Background: Annexin 5 is a member of the annexin family of calcium-dependent phospholipid binding proteins. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. In vitro studies have shown that Annexin 5 is a cellular protein which plays a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin (1). Through affinity assays Annexin 5 has been localized on cells that expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death (2). Annexin 5 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Apoptosis/Autophagy, Cancer, and Neurobiology research.

NCBI Accession: NM_001154

Pathways: Apoptosis