Annexin V Protein (His tag)
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- Target See all Annexin V (ANXA5) Proteins
- Annexin V (ANXA5) (Annexin A5 (ANXA5))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Annexin V protein is labelled with His tag.
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r40s
- Supplier
- Rockland
- Purpose
- Annexin 5 recombinant protein-HIS Epitope
- Purification
- Recombinant full-length human Annexin 5 Protein was expressed by E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.
- Purity
- >95%
- Top Product
- Discover our top product ANXA5 Protein
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- Application Notes
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Western_Blot_Dilution: User Optimized
Application_Note: Annexin 5 Protein is suitable for use in Western Blot. Expect a band approximately ~ 37 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 μg/μL
- Buffer
- Annexin 5 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 2 mM DTT, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- Annexin V (ANXA5) (Annexin A5 (ANXA5))
- Alternative Name
- ANXA5 (ANXA5 Products)
- Background
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Synonyms: ANXA5, PP4, ANX5, ENX2, Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, CBP-I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, PAP-I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, VAC-alpha
Background: Annexin 5 is a member of the annexin family of calcium-dependent phospholipid binding proteins. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. In vitro studies have shown that Annexin 5 is a cellular protein which plays a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin (1). Through affinity assays Annexin 5 has been localized on cells that expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death (2). Annexin 5 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Apoptosis/Autophagy, Cancer, and Neurobiology research.
- NCBI Accession
- NM_001154
- Pathways
- Apoptosis
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