ARTN Protein

Catalog No. ABIN6699531

Supplier Product No.: 009-001-u64-0020

Recombinant ARTN protein expressed in Escherichia coli (E. coli).

Quick Overview for ARTN Protein (ABIN6699531)

Target: ARTN (Artemin (ARTN))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 009-001-u64-0020

Supplier: Rockland

Purpose: Human Artemin Recombinant Protein

Purification: Artemin purity was determined to be greater than 98% as determined by analysis by HpLC and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependant proliferation of the SH-SY5Y cell line and is typically 4-8 ng/mL. The activity can also be determined by its ability to promote survival and neurite outgrowth.

Application Details

Application Notes:

Application Note: Recombinant human Artemin has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Artemin in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: ARTN (Artemin (ARTN))

Alternative Name: ARTN

Background:

Synonyms: ARTN, enovin, neublastin

Background: Artemin is a novel member of the glial cell line-derived neurotrophic factor (GNDF) ligand family. Current evidence suggests that Artemin signals through the receptor complex, GFRα3-RET, to influence neuron survival in vitro and in vivo. Recombinant human Artemin is a non-glycosylated, disulfide-linked homodimer, containing two 113 amino acid chains, with a total molecular weight of 24.2 kDa.

UniProt: Q5T4W7