BAG1 Protein (GST tag)

Catalog No. ABIN6699543

Supplier Product No.: 009-001-r46s

Recombinant BAG1 protein expressed in Escherichia coli (E. coli).

Quick Overview for BAG1 Protein (GST tag) (ABIN6699543)

Target: BAG1 (BCL2-Associated Athanogene (BAG1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >85%

Product Details

Purification tag / Conjugate: This BAG1 protein is labelled with GST tag.

Supplier Product No.: 009-001-r46s

Supplier: Rockland

Purpose: BAG1 recombinant protein-GST fusion protein

Purification: Recombinant human BAG1 (72-end) was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

Application Details

Application Notes:

Application Note: BAG1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. BAG1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 66 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: BAG1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: BAG1 (BCL2-Associated Athanogene (BAG1))

Alternative Name: BAG1

Background:

Synonyms: BAG family molecular chaperone regulator 1 (BAG-1) (Bcl-2-associated athanogene 1), HAP, RAP46

Background: BAG1 (also known as BCL2-associated athanogene) is a membrane protein rich in glutamic acid residues that binds to BCL2 and blocks apoptosis or programmed cell death (1). The BAG1-BCL2 complex enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms (2). Overexpression of BAG1 in 3T3 fibroblasts prevents apoptosis in the presence of low serum. BAG1 has also been shown to interact with activated glucocorticoid, androgen, estrogen and progesterone receptors. Binding to these receptors by BAG1 is dependent on receptor activation. BAG1 Protein is ideal for investigators involved in Signaling Proteins, Apoptosis Proteins, AKT/PKB Pathway, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, and Neurobiology research.

NCBI Accession: NM_004323