BID Protein (GST tag)

Catalog No. ABIN6699547

Supplier Product No.: 009-001-r51s

This Recombinant BID protein is produced in Insect cells (Sf9).

Quick Overview for BID Protein (GST tag) (ABIN6699547)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >85%

Product Details

Purification tag / Conjugate: This BID protein is labelled with GST tag.

Supplier Product No.: 009-001-r51s

Supplier: Rockland

Purpose: BID recombinant protein-GST fusion protein

Purification: Recombinant full-length human BID was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

Application Details

Application Notes:

Application Note: BID Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. BID Protein is suitable for use in Western Blot. Expect a band approximately ~ 52 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: BID Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BID

Background:

Synonyms: FP497, MGC42355, MGC15319, BH3-interacting domain death agonist, p22 BID, BH3-interacting domain death agonist p15, p15 BID, p13 BID, p11 BID

Background: BID is a BH3 interacting death domain that heterodimerizes with either agonist BAX or antagonist BCL2 (1). BID is a member of the BCL-2 family of cell death regulators and is a mediator of mitochondrial damage induced by caspase-8 (CASP8). BID initiates apoptosis by binding to regulatory sites on prosurvival BCL2 proteins to directly neutralize their function. Multiple alternatively spliced transcript variants of BID have been found, but the full-length nature of some variants has not been defined. BID together with Cathepsins play an important role in the actions of Camptothecin on breast cancer cells (2). BID Protein is ideal for investigators involved in Signaling Proteins, Apoptosis Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, and Neurobiology research.

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity