CAB39 Protein (GST tag)
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- Target See all CAB39 Proteins
- CAB39 (Calcium Binding Protein 39 (CAB39))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This CAB39 protein is labelled with GST tag.
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-s49s
- Supplier
- Rockland
- Purpose
- MO25 alpha recombinant protein-GST fusion protein
- Purification
- Recombinant full-length human MO25α was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.
- Purity
- >95%
- Top Product
- Discover our top product CAB39 Protein
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Calcium Binding Protein 39 (CAB39) (AA 1-341) protein (His tag)
custom-made CAB39 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Calcium Binding Protein 39 (CAB39) (AA 1-341) protein (His tag)custom-made CAB39 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Calcium Binding Protein 39 (CAB39) (AA 1-341) protein (His tag)CAB39 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Calcium Binding Protein 39 (CAB39) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CAB39 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Calcium Binding Protein 39 (CAB39) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)CAB39 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Calcium Binding Protein 39 (CAB39) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)CAB39 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Western_Blot_Dilution: User Optimized
Other: Kinase Assay-User Optimized
Application_Note: MO25α Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~63 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 μg/μL
- Buffer
- MO25α Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- CAB39 (Calcium Binding Protein 39 (CAB39))
- Alternative Name
- CAB39 (CAB39 Products)
- Background
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Synonyms: CAB39, CGI-66, FLJ22682, Calcium-binding protein 39 MO25alpha, Protein Mo25
Background: MO25α (mouse protein 25 alpha) is a 40- kDa protein that interacts with the STE20-related adaptor-alpha (STRADα) pseudo kinase to form a regulatory complex capable of stimulating the activity of the LKB1 tumor suppressor protein kinase (1). LKB1 plays a critical role in cell proliferation, polarity and energy metabolism. LKB1 is mutated in the inherited Peutz-Jeghers cancer syndrome (PJS). MO25α binds directly to a conserved Trp-Glu-Phe sequence at the STRADα C terminus, and markedly enhances the binding of STRADα to LKB1 thereby increasing LKB1 catalytic activity (2). MO25α Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cardiovascular Disease, and Metabolic Disorder research.
- NCBI Accession
- NM_016289
- Pathways
- AMPK Signaling
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