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CCL2 Protein

CCL2 Origin: Human Host: Escherichia coli (E. coli) Recombinant 98,00% SDS
Rockland
Catalog No. ABIN6699608
Supplier Product No.: 009-001-b60
  • Target See all CCL2 Proteins
    CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
    Protein Type
    Recombinant
    Origin
    • 16
    • 14
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 35
    • 10
    • 8
    • 2
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Supplier Product No.
    009-001-b60
    Supplier
    Rockland
    Purpose
    MCP-1 Human Recombinant Protein
    Purification
    Purity was determined to be greater than 98% as determined by analysis by RP-HPLC and by reducing and non- reducing SDS-PAGE, against known standard.
    Purity
    98,00%
    Endotoxin Level
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    MCP-1 activity is determined by the ability to chemoattract human monocytes or THP-1 cells at concentrations of 4 - 15 ng/mL.
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    Discover our top product CCL2 Protein
  • Application Notes

    Other: User Optimized

    Application_Note: MCP-1 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-MCP-1 in immunological assays.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 20 μL (20-200 μL)

    Buffer

    Buffer: 0.1 % Trifluoroacetic acid

    Stabilizer: None

    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Expiry Date
    6 months
  • Target
    CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
    Alternative Name
    CCL2 (CCL2 Products)
    Background

    Synonyms: CCL2, C-C motif chemokine 2, Monocyte Chemotactic and Activating Factor, MCAF, Small-inducible cytokine A2, SCYA2, Monocyte chemotactic protein 1, Monocyte secretory protein JE, HC11

    Background: MCP-1, or Monocyte chemoattractant protein 1, of the intercrine beta (chemokine CC) family, is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. It augments monocyte anti-tumor activity and has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. MCP-1 exists as a monomer or homodimer, in equilibrium. It binds to CCR2 and CCR4 and is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. Recombinant Human MCP-1 produced in E. coli is a non-glycosylated, polypeptide chain containing 76 amino acids and having a molecular mass of 8607 Daltons.

    UniProt
    P13500
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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