CCL20 Protein

Catalog No. ABIN6699618

Supplier Product No.: 010-001-b51

Recombinant CCL20 protein expressed in Escherichia coli (E. coli).

Quick Overview for CCL20 Protein (ABIN6699618)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 010-001-b51

Supplier: Rockland

Purpose: MIP-3α Mouse Recombinant Protein

Purification: Purity was determined to be greater than 95% by reducing and non- reducing SDS-PAGE and by analytical RP-HPLC.

Endotoxin Level: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: Mouse MIP-3-alpha is fully biologically active when compared to standard. Activity is determined by the proteins ability to chemoattract mouse CCR6 transfected HEK/293 cells using a concentration range of 0.1-10.0 ng/ml.

Application Details

Application Notes:

Application Note: MIP-3α protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-MIP-3α in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: Ccl20

Background:

Synonyms: C-C motif chemokine 20, Small-inducible cytokine A20, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, Liver and activation-regulated chemokine, CC chemokine LARC, Beta-chemokine exodus-1, CC chemokine ST38

Background: Recombinant Mouse MIP-3-alpha represents a CC chemokine that is expressed in the liver, lymph nodes, lung and other tissues and signals through the CCR6 receptor. Mouse MIP-3 alpha shows chemotactic activity towards lymphocytes and dendritic cells. Recombinant Mouse MIP-3 alpha has a molecular weight of 7.9 kDa, comprised of 70 amino acids.

UniProt: O89093

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection