CCL7 Protein

Catalog No. ABIN6699648

Supplier Product No.: 010-001-v76-0100

Recombinant CCL7 protein expressed in Escherichia coli (E. coli).

Quick Overview for CCL7 Protein (ABIN6699648)

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 010-001-v76-0100

Supplier: Rockland

Purpose: Mouse Monocyte Chemotactic Protein-3 (CCL7) Recombinant Protein

Purification: Monocyte Chemotactic protein-3 (CCL7) purity was determined to be greater than 98% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by its ability to chemoattract THP-1 cells at 10-100 ng/mL.

Application Details

Application Notes:

Application Note: Monocyte Chemotactic Protein-3 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Monocyte Chemotactic Protein-3 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: Ccl7

Background:

Synonyms: Intercrine/chemokine MARC, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3 (MCP-3), Protein FIC, Small-inducible cytokine A7

Background: Monocyte Chemotactic Protein 3 (MCP-3), also called CCL7, is produced by macrophages and some tumor cell lines. MCP-3 signals through three different G protein-coupled receptors, CCR1, CCR2, and CCR3. CCL7 chemoattracts monocytes and can regulate macrophage function. Recombinant mouse MCP-3 is a non-glycosylated protein, containing 74 amino acids, with a molecular weight of 8.5 kDa.

UniProt: Q03366