CCL8 Protein

Catalog No. ABIN6699655

Supplier Product No.: 009-001-v75-0010

This Recombinant CCL8 protein is produced in Escherichia coli (E. coli).

Quick Overview for CCL8 Protein (ABIN6699655)

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 009-001-v75-0010

Supplier: Rockland

Purpose: Human Monocyte Chemotactic Protein-2 (CCL8) Recombinant Protein

Purification: Monocyte Chemotactic protein-2 (CCL8) purity was determined to be greater than 98% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to chemoattract human PBMCs and is typically in the range of 10-100 ng/mL.

Application Details

Application Notes:

Application Note: Monocyte Chemotactic Protein-2 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Monocyte Chemotactic Protein-2 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Alternative Name: CCL8

Background:

Synonyms: HC14, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2 (MCP-2), Small-inducible cytokine A8

Background: Monocyte Chemotactic Protein 2 (MCP-2), also known as CCL8, is implicated in allergic responses through its ability to activate mast cells, eosinophils, and basophils. MCP-2 is known to signal through several G protein-coupled receptors including, CCR1, CCR2B, and CCR5. Recombinant human MCP-2 is a non-glycosylated protein, containing 76 amino acids, with a molecular weight of 8.9 kDa.

UniProt: P80075