CXCL1 Protein

Catalog No. ABIN6699668

Supplier Product No.: 010-001-v15-0100

This Recombinant CXCL1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CXCL1 Protein (ABIN6699668)

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 97,00%

Product Details

Supplier Product No.: 010-001-v15-0100

Supplier: Rockland

Purpose: Mouse Gro-alpha /KC (CXCL1) Recombinant Protein

Purification: Gro-alpha /KC (CXCL1) purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by its ability to chemoattract human neutrophils cells and is typically 10 -100 ng/mL.

Application Details

Application Notes:

Application Note: Gro Alpha Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Gro Alpha in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Alternative Name: Cxcl1

Background:

Synonyms: C-X-C motif chemokine 1, MGSAα, mKC, NAP-3, Platelet-derived growth factor-inducible protein KC, GRO1, rCINC, KC, Secretory protein N51

Background: GROα, also known as CXCL1, is a chemokine thought to have mitogenic properties and chemoattract neutrophils. Secreted by macrophages, epithelial cells, neutrophils and melanomas, GROα signals through chemokine receptor, CXCR2, and has been implicated in the processes of spinal cord formation, inflammation, angiogenesis, tumorigenesis, and wound healing. Recombinant mouse GROα is a non-glycosylated protein, containing 72 amino acids, with a molecular weight of 7.8 kDa.

UniProt: P12850

Pathways: Autophagy