CXCL10 Protein

Catalog No. ABIN6699676

Supplier Product No.: 010-001-v69-0100

This Recombinant CXCL10 protein is produced in Escherichia coli (E. coli).

Quick Overview for CXCL10 Protein (ABIN6699676)

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 97,00%

Product Details

Supplier Product No.: 010-001-v69-0100

Supplier: Rockland

Purpose: Mouse IP-10 (CXCL10) Recombinant Protein

Purification: Ip-10 (CXCL10) purity was determined to be greater than 97% as determined by HpLC and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by its ability to chemoattract primary human T cells at 0.1-10 ng/mL.

Application Details

Application Notes:

Application Note: IP-10 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-IP-10 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Alternative Name: Cxcl10

Background:

Synonyms: 10 kDa interferon gamma-induced protein (IP-10), C7, Interferon-gamma induced protein CRG-2, small-inducible cytokine B10

Background: The chemokine IP-10 (or CXCL10) is a chemokine made by monocytes, endothelial cells and fibroblasts in response to treatment with IFNγ. IP-10 functions as a chemoattractant to cells expressing the G protein-coupled receptor, CXCR3, which is found mainly on activated T cells and NK cells. IP-10 plays an important role in Th1 type inflammatory diseases and autoimmune diseases such as, Hashimoto's thyroiditis, Graves' disease and Type 1 diabetes mellitus. Recombinant mouse IP-10 is a non-glycosylated protein, containing 77 amino acids, with a molecular weight of 8.7 kDa.

UniProt: P17515