CX3CL1 Protein

Catalog No. ABIN6699702

Supplier Product No.: 009-001-h49-0005

Recombinant CX3CL1 protein expressed in Escherichia coli (E. coli).

Quick Overview for CX3CL1 Protein (ABIN6699702)

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: 98,00%

Product Details

Supplier Product No.: 009-001-h49-0005

Supplier: Rockland

Purpose: Human Fractalkine (CX3CL1) Recombinant Protein

Purification: Fractalkine (CX3CL1) purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose dependent chemotaxis of human PBMCs and is typically starting between 10-100 ng/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: Fractalkine Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Fractalkine in immunological assays.

Comment:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Alternative Name: CX3CL1

Background:

Synonyms: C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1

Background: Fractalkine, also known as CX3CL1, is an atypical chemokine that was the first of a fourth chemokine motif (CX3C). It is thought to function as a T cell and monocyte chemotractant and is produced by non-haemopoietic cells. Fractalkine is made in a soluble and membrane bound form in activated endothelial cells which is thought to promote adhesion of leukocytes. Recombinant human Fractalkine is a non-glycosylated protein, containing 76 amino acids, with a molecular weight of 8.6 kDa.

UniProt: P78423

Pathways: Synaptic Membrane