M-CSF/CSF1 Protein

Catalog No. ABIN6699709

Supplier Product No.: 009-001-v78-0010

This Recombinant M-CSF/CSF1 protein is produced in Escherichia coli (E. coli).

Quick Overview for M-CSF/CSF1 Protein (ABIN6699709)

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 97,00%

Product Details

Supplier Product No.: 009-001-v78-0010

Supplier: Rockland

Purpose: Human Macrophage Colony Stimulating Factor Recombinant Protein

Purification: Macrophage Colony Stimulating Factor purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of mouse NFS-60 cells and is typically 10.0 ng/mL.

Application Details

Application Notes:

Application Note: Macrophage Colony Stimulating Factor Recombinant Protein has been tested by SDS-PAGE and biological activity and s suitable as a control for polyclonal or monoclonal anti-Macrophage Colony Stimulating Factor in immunological assays. Lyophilized in 10 mM sodium phosphate, 100 mM sodium chloride, pH 8.0.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer: Lyophilized in 10 mM sodium phosphate, 100 mM sodium chloride, pH 8.0.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Alternative Name: CSF1

Background:

Synonyms: MGI-IM, Lanimostim

Background: Macrophage Colony Stimulating Factor (M-CSF) is a hematopoietic growth factor produced by a wide variety of cells. M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells. Recombinant human M-CSF is a disulfide linked homodimer, containing two 159 amino acid chains, with a total molecular weight of 36.8 kDa.

UniProt: P09603

Pathways: RTK Signaling