G-CSF Protein

Catalog No. ABIN6699717

Supplier Product No.: 009-001-u93-0010

Recombinant G-CSF protein expressed in Escherichia coli (E. coli).

Quick Overview for G-CSF Protein (ABIN6699717)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 009-001-u93-0010

Supplier: Rockland

Purpose: Human Granulocyte Colony Stimulating Factor Recombinant Protein

Purification: Granulocyte Colony Stimulating Factor purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of mouse NFS-60 and is typically 10-60 pg/mL.

Application Details

Application Notes:

Application Note: Granulocyte Colony Stimulating Factor Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Granulocyte Colony Stimulating Factor in immunological assays. Lyophilized in 20 mM acetic acid, 50 nM sodium chloride.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer: Lyophilized in 20 mM acetic acid, 50 nM sodium chloride.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: CSF3

Background:

Synonyms: CSF-3, MGI-1G, GM-CSFβ, pluripoietin

Background: Granulocyte-Colony Stimulating Factor (G-CSF) is a growth factor that is considered the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF, while in epithelial, endothelial, and fibroblastic cells, secretion of G-CSF is induced by IL-17. Human and mouse G-CSF are cross-reactive. Recombinant human G-CSF is a non-glycosylated protein, containing 175 amino acids, with a molecular weight of 18.8 kDa.

UniProt: P09919

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization