Cyclin E1 Protein (CCNE1) (GST tag)

Catalog No. ABIN6699743

Supplier Product No.: 009-001-r79s

This Recombinant Cyclin E1 protein is expressed in Insect cells (Sf9).

Quick Overview for Cyclin E1 Protein (CCNE1) (GST tag) (ABIN6699743)

Target: Cyclin E1 (CCNE1)

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >90%

Product Details

Purification tag / Conjugate: This Cyclin E1 protein is labelled with GST tag.

Supplier Product No.: 009-001-r79s

Supplier: Rockland

Purpose: CyclinE1 recombinant protein-GST fusion protein

Purification: Recombinant full-length human CyclinE1 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Application Details

Application Notes:

Application Note: CyclinE1 is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. CyclinE1 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~ 73 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Other: Kinase Assay-User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: CyclinE1 is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Cyclin E1 (CCNE1)

Alternative Name: CCNE1

Background:

Synonyms: CCNE1, CCNE, G1/S-specific cyclin-E1

Background: Cyclin E1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle that functions as regulators of CDK kinases. Cyclin E1 activity is required for centrosome duplication during S phase and this mechanism could coordinate centrosome reproduction with cycles of DNA synthesis and mitosis (1). The downregulation of cyclin E-CDK2 kinase activity following the G1/S-phase transition that is necessary for the maintenance of karyotypic stability. Cyclin E also has a modular centrosomal-targeting domain which is essential for promoting S phase entry in a Cdk2-independent manner (2). Cyclin E1 Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.

NCBI Accession: NM_001238

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases