Cyclin H Protein (CCNH) (GST tag)

Catalog No. ABIN6699745

Supplier Product No.: 009-001-r80s

This Recombinant Cyclin H protein is produced in Insect cells (Sf9).

Quick Overview for Cyclin H Protein (CCNH) (GST tag) (ABIN6699745)

Target: Cyclin H (CCNH)

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >90%

Product Details

Purification tag / Conjugate: This Cyclin H protein is labelled with GST tag.

Supplier Product No.: 009-001-r80s

Supplier: Rockland

Purpose: CyclinH recombinant protein-GST fusion protein

Purification: Recombinant full length human CyclinH was expressed in insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Application Details

Application Notes:

Application Note: CyclinH Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. CyclinH Protein is suitable for use in Western Blot. Expect a band approximately ~ 39 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: CyclinH Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Cyclin H (CCNH)

Alternative Name: CCNH

Background:

Synonyms: CCNH, CAK, p34, p37, Cyclin-H, MO15-associated protein

Background: Cyclin H is related to the highly conserved cyclin family. Cyclin family members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Cyclin H forms a complex with CDK7 kinase and the ring finger protein MAT1 which is able to phosphorylate CDK2 and CDC2 kinases and thus act as a CDK-activating kinase (CAK) (1). Cdk-activating kinase complex is a component of human transcription factor TFIIH and plays an important role in the processes of transcription, DNA repair, and cell cycle progression (2). Cyclin H Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.

NCBI Accession: NM_001239

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase