Cyclin K Protein (CCNK) (GST tag)

Catalog No. ABIN6699747

Supplier Product No.: 009-001-r81s

Recombinant Cyclin K protein expressed in Insect cells (Sf9).

Quick Overview for Cyclin K Protein (CCNK) (GST tag) (ABIN6699747)

Target: Cyclin K (CCNK)

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >85%

Product Details

Purification tag / Conjugate: This Cyclin K protein is labelled with GST tag.

Supplier Product No.: 009-001-r81s

Supplier: Rockland

Purpose: CyclinK recombinant protein-GST fusion protein

Purification: Recombinant full-length human CyclinK was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

Application Details

Application Notes:

Application Note: CyclinK Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. CyclinK Protein is suitable for use in Western Blot. Expect a band approximately ~ 67 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: CyclinK Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Cyclin K (CCNK)

Alternative Name: CCNK

Background:

Synonyms: CCNK, CPR4, MGC9113, Cyclin-K

Background: CyclinK is a member of the cyclin family which can regulate transcription through their association with and activation of cyclin-dependent kinases (CDK). The CDK/CyclinK complex can phosphorylate the C-terminal domain (CTD) of the large subunit of RNA polymerase II (1). CyclinK is associated with potent CTD kinase and CDK kinase activities in vitro and coimmunoprecipitates with POLR2A. CyclinK is a new member of the transcription cyclin family which may play a dual role in regulating CDK and RNA polymerase II activities (2). Cyclin K Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.