Cyclin Y Protein (CCNY) (GST tag)

Catalog No. ABIN6699751

Supplier Product No.: 009-001-r83s

This Recombinant Cyclin Y protein is produced in Insect cells (Sf9).

Quick Overview for Cyclin Y Protein (CCNY) (GST tag) (ABIN6699751)

Target: Cyclin Y (CCNY)

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >95%

Product Details

Purification tag / Conjugate: This Cyclin Y protein is labelled with GST tag.

Supplier Product No.: 009-001-r83s

Supplier: Rockland

Purpose: CyclinY recombinant protein-GST fusion protein

Purification: Recombinant human Cyclin Y (2-end) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Application Details

Application Notes:

Application Note: CyclinY Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. CyclinY Protein is suitable for use in Western Blot. Expect a band approximately ~ 65 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: CyclinY Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Cyclin Y (CCNY)

Alternative Name: CCNY

Background:

Synonyms: CCNY, C10orf9, CBCP1, CCNX, CFP1, Cyclin-Y, Cyc-Y, Cyclin box protein 1, Cyclin fold protein 1, cyclin-X

Background: Cyclin Y belongs to the highly conserved cyclin family. The members of the cyclin family are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclin Y plays an important role in the processes of transcription, DNA repair, and cell cycle progression. Cyclin Y regulates cyclin-dependent kinases (1). Cyclin Y is an isoform of CCNY that is also known as CFP1 variant A which has a calculated molecular mass of 36.9 kD. (2). Cyclin Y specifically activates transcription through the MYC promoter. Cyclin Y Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.

NCBI Accession: NM_145012