FGF21 Protein

Catalog No. ABIN6699851

Supplier Product No.: 009-001-u77-0020

This Recombinant FGF21 protein is expressed in Escherichia coli (E. coli).

Quick Overview for FGF21 Protein (ABIN6699851)

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 009-001-u77-0020

Supplier: Rockland

Purpose: Human Fibroblast Growth Factor-21 Recombinant Protein

Purification: Fibroblast Growth Factor-21 purity was determined to be greater than 95% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of NIH 3T3 cells in the presence of 5 μg/mL FGF binding protein and is typically 0.12-0.6 μg/mL.

Application Details

Application Notes:

Application Note: Fibroblast Growth Factor-21 Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Fibroblast Growth Factor-21 in immunological assays. Lyophilized in 0.1M Sodium Chloride and 0.01M Sodium Phosphate, pH 7.5.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer: Lyophilized in 0.1M Sodium Chloride and 0.01M Sodium Phosphate, pH 7.5.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Alternative Name: FGF21

Background:

Synonyms: FGFL

Background: Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. FGF-21 is most closely related to FGF-19 and shows low binding to heparin and is highly diffusible. Recombinant human FGF-21 is a non-glycosylated protein, containing 182 amino acids, with a molecular weight of 19.5 kDa.

UniProt: Q9NSA1

Pathways: RTK Signaling