FLT3LG Protein

Catalog No. ABIN6699871

Supplier Product No.: 009-f01-u86-0010

This Recombinant FLT3LG protein is produced in Escherichia coli (E. coli).

Quick Overview for FLT3LG Protein (ABIN6699871)

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Grade: Animal-Free

Product Details

Supplier Product No.: 009-f01-u86-0010

Supplier: Rockland

Purpose: Human Flt-3 Ligand Recombinant Protein (Animal Free)

Purification: FMS-related Tyrosine Kinase 3 Ligand is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 98% as determined by analysis by HPLC, UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-PAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent production of OCI-AML5 cell proliferation and is typically 10 ng/mL.

Application Details

Application Notes:

Application Note: Flt-3 Ligand Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti- Flt-3 Ligand in immunological assays. Buffer Formulation: 10 mM sodium phosphate and 50 mM sodium chloride, pH 7.5.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer: Buffer Formulation: 10 mM sodium phosphate and 50 mM sodium chloride, pH 7.5.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Alternative Name: FLT3LG

Background:

Synonyms: FLt3 L, Fms-related tyrosine kinase 3 ligand

Background: FMS-related Tyrosine Kinase 3 Ligand (FLT-3 Ligand) is a growth factor important for the proliferation of hematopoietic cells. FLT-3 Ligand binds to, and transmits signals through, the receptor tyrosine kinase known as FMS-like Tyrosine Kinase-3 (FLT-3). FLT-3 Ligand promotes long-term expansion and differentiation of human pro-B cells in the presence of IL-7 or in combination of IL-7 and IL-3. Human FLT-3 Ligand can stimulate the proliferation of cells expressing murine FLT-3 receptors. Recombinant human FLT-3 Ligand is a non-glycosylated protein, containing 155 amino acids, with a molecular weight of 17.7 kDa.

UniProt: P49771

Pathways: RTK Signaling