IL17F Protein

Catalog No. ABIN6700053

Supplier Product No.: 009-001-b31

Recombinant IL17F protein expressed in Escherichia coli (E. coli).

Quick Overview for IL17F Protein (ABIN6700053)

Target: IL17F (Interleukin 17F (IL17F))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 97,00%

Product Details

Supplier Product No.: 009-001-b31

Supplier: Rockland

Purpose: IL-17F Human Recombinant Protein

Purification: Purity was determined to be approximately 97% by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.

Endotoxin Level: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: The ED50 of recombinant human IL-17F, as determined by induction of IL-6 in NIH3T3 cells, is 10.18 ng/mL.

Application Details

Application Notes:

Application Note: IL-17F protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-17F in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 25 μL (25-250 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: IL17F

Background:

Synonyms: Interleukin-17F cytokine, IL-17F, Interleukin-24, IL-24, Cytokine ML-1

Background: Recombinant Human Interleukin-17-F is a non-glycosylated protein consisting of two 134 amino acid polypeptide chains that are cysteine linked homodimeric polypeptide chains with a molecular weight of 30 kDa. Homodimeric interleukin-17F is a member of the IL-17 family of six homodimeric proteins (IL-17A-17F). Activity studies reveal IL-17A to be the most potent, followed by IL-17A/F and IL-17F 100 fold lower than IL-17A.

UniProt: Q96PD4

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity