IL17F Protein

Catalog No. ABIN6700054

Supplier Product No.: 010-001-b31

This Recombinant IL17F protein is produced in Escherichia coli (E. coli).

Quick Overview for IL17F Protein (ABIN6700054)

Target: IL17F (Interleukin 17F (IL17F))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 97,00%

Product Details

Supplier Product No.: 010-001-b31

Supplier: Rockland

Purpose: IL-17F Mouse Recombinant Protein

Purification: Purity was determined to be greater than 97% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.

Endotoxin Level: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: IL-17F is fully bioactive when compared to standard. The ED50 as determined by the dose-dependant induction of IL-6 in NHDF is 1.94 ng/ml.

Application Details

Application Notes:

Application Note: IL-17F protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-17F in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 25 μL (25-250 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: Il17f

Background:

Synonyms: Interleukin-17F cytokine, IL-17F, Interleukin-24, IL-24, Cytokine ML-1

Background: Recombinant Mouse IL-17F produced in E.coli is a homodimeric, non-glycosylated polypeptide chain, the monomer containing a total of 133 amino acids and having a molecular mass of 14.9 kDa. Homodimeric interleukin-17F is a member of the IL-17 family of six homodimeric proteins (IL-17A-17F). Activated CD4+ T cells have also been shown to produce Mouse IL-17F. Activity studies reveal IL-17A to be the most potent, followed by IL-17A/F and IL-17F 100 fold lower than IL-17A. Recombinant Mouse IL-17F produced in E.Coli is a non-glycosylated polypeptide comprised of two monomeric subunit each of mIL-17F. The dimer consists of 266 amino acids, with an approximate molecular weight of 29.8 kDa.

UniProt: Q7TNI7

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity