IL-3 Protein

Catalog No. ABIN6700109

Supplier Product No.: 009-001-b92

Product Details

Target: IL-3 (Interleukin 3 (IL-3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 009-001-b92

Supplier: Rockland

Purpose: IL-3 Recombinant Human Protein

Purification: Purity was determined to be greater than 95% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE, against known standard.

Purity: 95,00%

Endotoxin Level: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: Human IL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of TF-1 cells is less then 0.1 ng/ml.

Application Details

Application Notes:

Other: User Optimized

Application_Note: IL-3 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-3 in immunological assays.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer: Buffer formation: 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL3 (IL-3 Products)

Background:

Synonyms: Interleukin-3 cytokine, IL-3, Multipotential colony-stimulating factor, Hematopoietic growth factor, P-cell-stimulating factor, Mast cell growth factor, MCGF

Background: Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system and appears to be important in several chronic inflammatory diseases. Recombinant Human IL-3 produced in E. coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15,000 Daltons.

UniProt: P08700

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy