IL-31 Protein

Catalog No. ABIN6700122

Supplier Product No.: 010-001-v66-0010

Recombinant IL-31 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-31 Protein (ABIN6700122)

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 97,00%

Product Details

Supplier Product No.: 010-001-v66-0010

Supplier: Rockland

Purpose: Mouse Interleukin IL-31 Recombinant Protein

Purification: Interleukin IL-31 purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose dependent production of IL-8 from human PBMCs and is typically less than 25 ng/mL.

Application Details

Application Notes:

Application Note: Interleukin IL-31 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin IL-31 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Alternative Name: Il31

Background:

Synonyms: Interleukin-31, IL-31, Interleukin 32

Background: Interleukin-31 (IL-31) is an immunoregulatory cytokine that is mainly expressed by activated TH2 cells. IL-31 signals through the IL-31 Receptor (IL-31RA) and the oncostain receptor (OSMR), which are expressed on monocytes, epithelial cells, and keratinocytes. IL-31 has a role in inflammatory skin diseases. Recombinant mouse IL-31 is a non-glycosylated protein, containing 141 amino acids, with a molecular weight of 15.7 kDa.

UniProt: Q6EAL8