IL-4 Protein

Catalog No. ABIN6700140

Supplier Product No.: 010-001-b93-0005

Recombinant IL-4 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-4 Protein (ABIN6700140)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 010-001-b93-0005

Supplier: Rockland

Purpose: Mouse Interleukin-4 Recombinant Protein

Purification: Interleukin-4 purity was determined to be greater than 95% as determined by HpLC and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by dose-dependent induction of HT-2 cell proliferation and is typically less than 2 ng/mL.

Application Details

Application Notes:

Application Note: Interleukin-4 Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-4 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: Il4

Background:

Synonyms: B cell Stimulating Factor 1 (BSF-1), BCDF, BCGF, B-cell IgG differentiation factor, B-cell growth factor 1, IGG1 induction factor, Lymphocyte stimulatory factor 1

Background: Interleukin-4 (IL-4) is an immuno-modulatory cytokine, produced primarily by a subgroup of helper T cells, known as Th2. IL-4 is known to induce naive helper T cells to differentiate to Th2 cells, which subsequently produce more IL-4. Th2 cells regulate a wide variety of other immune cells including, B cells, where it has the ability to promote IgG to IgE isotype switching. Recombinant mouse IL-4 is a non-glycosylated protein, containing 121 amino acids, with a molecular weight of 13.5 kDa.

UniProt: P07750

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation