KIT Ligand Protein (KITLG)

Catalog No. ABIN6700189

Supplier Product No.: 009-001-w20-0100

This Recombinant KIT Ligand protein is produced in Escherichia coli (E. coli).

Quick Overview for KIT Ligand Protein (KITLG) (ABIN6700189)

Target: KIT Ligand (KITLG)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 009-001-w20-0100

Supplier: Rockland

Purpose: Human Stem Cell Factor Recombinant Protein

Purification: Stem Cell Factor purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-PAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent stimulation of Human TF-1 cells and is typically 15 ng/mL.

Application Details

Application Notes:

Application Note: Stem Cell Factor Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Stem Cell Factor in immunological assays. Buffer Formulation: 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 1.0 mL

Concentration: 0.1 mg/mL

Buffer: Buffer Formulation: 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20°C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: KIT Ligand (KITLG)

Alternative Name: KITLG

Background:

Synonyms: c-Kit Ligand, KL, Steel Factor, Stem cell factor (SCF), Mast cell growth factor (MGF)

Background: Stem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance or survival of hematopoietic stem cells. Human SCF shows no activity on murine cells, but murine and rat SCF are active on human cells. Recombinant human SCF is a non-glycosylated protein, containing 165 amino acids, with a molecular weight of 18.4 kDa

UniProt: P21583

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway