Myostatin Propeptide Protein

Catalog No. ABIN6700280

Supplier Product No.: 009-001-w02-0100

This Recombinant protein is expressed in Escherichia coli (E. coli).

Quick Overview for Myostatin Propeptide Protein (ABIN6700280)

Target: Myostatin Propeptide

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 009-001-w02-0100

Supplier: Rockland

Purpose: Human Myostatin Propeptide Recombinant Protein

Purification: Myostatin propeptide purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by its ability to inhibit 50 ng/mL of Myostatin on MPC-11 cells and is typically 0.01-0.04 μg/mL.

Application Details

Application Notes:

Application Note: Myostatin Propeptide Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Myostatin Propeptide in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: 0.02M HCl

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: Myostatin Propeptide

Background:

Synonyms: Myostatin

Background: Myostatin (GDF-8), a member of the TGF-β superfamily, is a potent and specific negative regulator of skeletal muscle mass. The myostatin propeptide is known to bind and inhibit myostatin in vitro. This interaction is relevant in vivo, with a majority (>70 % ) of myostatin in serum bound to its propeptide acting as a negative regulator of myostatin. Recombinant human Myostatin Propeptide is a non-glycosylated protein, containing 244 amino acids, with a molecular weight of 27.8 kDa.

UniProt: O08689