SMAD5 Protein (GST tag)

Catalog No. ABIN6700549

Supplier Product No.: 009-001-t41s

This Recombinant SMAD5 protein is produced in Escherichia coli (E. coli).

Quick Overview for SMAD5 Protein (GST tag) (ABIN6700549)

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >85%

Product Details

Purification tag / Conjugate: This SMAD5 protein is labelled with GST tag.

Supplier Product No.: 009-001-t41s

Supplier: Rockland

Purpose: SMAD5 recombinant protein-GST fusion protein

Purification: Recombinant full-length human SMAD5 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

Application Details

Application Notes:

Application Note: SMAD5 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. SMAD5 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~82 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Other: Kinase Assay-User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: SMAD5 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Alternative Name: SMAD5

Background:

Synonyms: Dwfc, JV5-1, MADH5, DKFZp781C1895, DKFZp781O1323, Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5

Background: SMAD5 is a member of the SMAD family and mediates signaling by the transforming growth factor-beta (TGFβ) superfamily and related ligands (1). SMAD5 plays a critical role in the signaling pathway by which TGFβ inhibits the proliferation of human hematopoietic progenitor cells. SMAD5 is up-regulated in gastric epithelial cells during the infection of the pathogen Helicobacter pylori and it mediates apoptosis of gastric epithelial cells induced by H. pylori infection (2). In mature human B cells, bone morphogenetic protein 6 (BMP-6) inhibits cell growth and rapidly induces phosphorylation of SMADs 5 and 8. SMAD5 Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, AKT/PKB Pathway, Angiogenesis, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Inflammation, JAK/STAT Pathway, JNK/SAPK Pathway, NfkB Pathway, and WNT Signaling research.

NCBI Accession: NM_005903