14-3-3 sigma/SFN Protein (GST tag)

Catalog No. ABIN6700573

Supplier Product No.: 009-001-r31s

This Recombinant 14-3-3 sigma/SFN protein is produced in Escherichia coli (E. coli).

Quick Overview for 14-3-3 sigma/SFN Protein (GST tag) (ABIN6700573)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >90%

Product Details

Purification tag / Conjugate: This 14-3-3 sigma/SFN protein is labelled with GST tag.

Supplier Product No.: 009-001-r31s

Supplier: Rockland

Purpose: 14-3-3 sigma recombinant protein-GST fusion protein

Purification: Recombinant full-length human 14-3-3σ was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Application Details

Application Notes:

Application Note: 14-3-3σ Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. 14-3-3σ Protein is suitable for use in Western Blot. Expect a band approximately ~ 51 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: 14-3-3σ Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: YWHAS

Background:

Synonyms: 14-3-3 sigma, SFN, stratifin, YWHAS

Background: 14-3-3σ or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents (1). The induction of 14-3-3σ is mediated by a p53 responsive element. Exogenous introduction of 14-3-3σ into cycling cells results in a G2 cell cycle arrest (2). Knockout of 14-3-3σ in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3σ -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3σ -deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3σ plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death. 14-3-3σ Protein is ideal for investigators involved in Cell Stress& Chaperone Proteins, AKT/PKB Pathway, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, and WNT Signaling research.

NCBI Accession: NM_006826

Pathways: p53 Signaling, Myometrial Relaxation and Contraction