TAF1 Protein (N-Term) (GST tag)

Catalog No. ABIN6700605

Supplier Product No.: 009-001-t71s

Recombinant TAF1 protein expressed in Insect cells (Sf9).

Quick Overview for TAF1 Protein (N-Term) (GST tag) (ABIN6700605)

Target: TAF1 (TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >70%

Product Details

Protein Characteristics: N-Term

Purification tag / Conjugate: This TAF1 protein is labelled with GST tag.

Supplier Product No.: 009-001-t71s

Supplier: Rockland

Purpose: TAF1 (N-term) recombinant protein-GST fusion protein

Purification: Recombinant human TAF1 (1-561) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

Application Details

Application Notes:

Application Note: TAF1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. TAF1 Protein is suitable for use in Western Blot. Expect a band approximately ~130 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: TAF1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: TAF1 (TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1))

Alternative Name: TAF1

Background:

Synonyms: BA2R, CCG1, CCGS, DYT3, DYT3/TAF1, KAT4, N-TAF1, NSCL2, OF, P250, TAF2A, Transcription initiation factor TFIID subunit 1

Background: TAF1 is a DNA-binding protein complex required for RNA polymerase II-mediated transcription of many, if not all, protein-encoding genes in eukaryotic cells. TAF1 plays a key role in the initiation process, since it binds to the TATA element to form a complex that nucleates the assembly of the other components into a preinitiation complex and that may be stable through multiple rounds of transcription. TAF1 may be targeted to specific chromatin-bound promoters and may play a key role in chromatin recognition (1). TAF1 also serves to link the control of transcription to the cell cycle (2). TAF1 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cell Cycle, and Invasion/Metastasis research.

NCBI Accession: NM_138923