UBE2Z Protein (His tag)

Catalog No. ABIN6700838

Supplier Product No.: 009-001-u47s

This Recombinant UBE2Z protein is expressed in Escherichia coli (E. coli).

Quick Overview for UBE2Z Protein (His tag) (ABIN6700838)

Target: UBE2Z (Ubiquitin-Conjugating Enzyme E2Z (UBE2Z))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >80%

Product Details

Purification tag / Conjugate: This UBE2Z protein is labelled with His tag.

Supplier Product No.: 009-001-u47s

Supplier: Rockland

Purpose: UBE2Z (USE1) recombinant protein-HIS Epitope

Purification: Recombinant human UBE2Z (USE1) (amino acids 109-end) protein was expressed by E. coli cells using an N-terminal his epitope. The purity was determined to be >80% by densitometry.

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Application_Note: UBEZ Protein is suitable for use in Western Blot. Expect a band approximately ~30 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: UBE2Z Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: UBE2Z (Ubiquitin-Conjugating Enzyme E2Z (UBE2Z))

Alternative Name: UBE2Z

Background:

Synonyms: HOYS7, USE1, Ubiquitin-conjugating enzyme E2 Z, Uba6-specific E2 conjugating enzyme 1, Use1, Ubiquitin carrier protein Z, Ubiquitin-protein ligase Z

Background: UBE2Z (USE1) encodes the ubiquitin conjugating enzyme (E2) which is involved in Ubiquitination, the covalent attachment of the protein ubiquitin (Ub) to other cellular proteins (1). UBE2Z plays an important role in the ubiquitin process and has been implicated in a number of important physiological processes including apoptosis. UBE2Z is specifically charged with ubiquitin by UBA6 (2). UBE2Z is widely expressed in human tissues and expression is especially high in placenta, pancreas, spleen and testis. UBE2Z Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Cancer, Cell Cycle, and Neurobiology research.

NCBI Accession: NM_023079