MOS Protein (GST tag)

Catalog No. ABIN6700848

Supplier Product No.: 009-001-s52s

Recombinant MOS protein expressed in Insect cells (Sf9).

Quick Overview for MOS Protein (GST tag) (ABIN6700848)

Target: MOS (Moloney Sarcoma Oncogene (MOS))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >70%

Product Details

Purification tag / Conjugate: This MOS protein is labelled with GST tag.

Supplier Product No.: 009-001-s52s

Supplier: Rockland

Purpose: MOS recombinant protein-GST fusion protein

Purification: Recombinant full-length human MOS was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

Application Details

Application Notes:

Application Note: MOS Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. MOS Protein is suitable for use in Western Blot. Expect a band approximately ~66 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: MOS Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: MOS (Moloney Sarcoma Oncogene (MOS))

Alternative Name: MOS

Background:

Synonyms: MGC119962, MGC119963, MSV, Proto-oncogene serine/threonine-protein kinase mos EC 2.7.11.1, Oocyte maturation factor mos, Proto-oncogene c-Mos

Background: MOS or v-mos Moloney murine sarcoma viral oncogene homolog is a serine/threonine kinase that activate the MAP kinase cascade through direct phosphorylation of the MAP kinase activator MEK (1). The early site-specific phosphorylation of CPEB , which was catalyzed by Eg2 is essential for the polyadenylation of c-mos mRNA, subsequent translation of c-mos, and oocyte maturation (2). The interaction with and phosphorylation of MYOD by MOS enhances muscle differentiation. MOS is an essential component of the cytostatic factor that acts almost exclusively in the second meiotic metaphase arrest. MOS Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cell Cycle, and Ser/Thr Kinases research.

NCBI Accession: NM_005372