Vimentin Protein (VIM) (His tag)

Catalog No. ABIN6700879

Supplier Product No.: 009-001-e54s

This Recombinant Vimentin protein is produced in Escherichia coli (E. coli).

Quick Overview for Vimentin Protein (VIM) (His tag) (ABIN6700879)

Target: Vimentin (VIM)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >95%

Product Details

Purification tag / Conjugate: This Vimentin protein is labelled with His tag.

Supplier Product No.: 009-001-e54s

Supplier: Rockland

Purpose: Vimentin recombinant protein-HIS Epitope

Purification: Recombinant full-length human Vimentin was expressed by E. coli cells using an N-terminal his epitope. The purity was determined to be >95% by densitometry.

Application Details

Application Notes:

Application Note: Vimentin Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. Vimentin Protein is suitable for use in Western Blot. Expect a band approximately ~60 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: Vimentin Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Vimentin (VIM)

Alternative Name: VIM

Background:

Synonyms: VIM, FLJ36605, Vimentin

Background: Vimentin is a member of intermediate filament family of proteins and is an important structural feature of eukaryotic cells (1). Along with microtubules and actin microfilaments, Vimentin make up the cytoskeleton component of cells. Studies have shown that Vimentin is attached to the nucleus, endoplasmic reticulum and mitochondria, either laterally or terminally. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol (2). Thus, Vimentin plays a key role in maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vimentin Protein is ideal for investigators involved in Signaling Proteins, Microtubule/Actin Associated Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Inflammation, Invasion/Metastasis, and Neurobiology research.

NCBI Accession: NM_003380

Pathways: Caspase Cascade in Apoptosis