GDNF Protein

Catalog No. ABIN6700947

Supplier Product No.: 012-001-v10-0010

Recombinant GDNF protein expressed in Escherichia coli (E. coli).

Quick Overview for GDNF Protein (ABIN6700947)

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 012-001-v10-0010

Supplier: Rockland

Purpose: Rat Glial Derived Neurotrophic Factor Recombinant Protein

Purification: Glial Derived Neurotrophic Factor purity was determined to be greater than 98% as determined by analysis by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of C6 cells and is typically less than 0.1 μg/mL.

Application Details

Application Notes:

Application Note: Glial Derived Neurotrophic Factor Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Glial Derived Neurotrophic Factor in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Alternative Name: Gdnf

Background:

Synonyms: ATF-1

Background: Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a neurotrophic factor that is closely related to other neurotrophic factors, such as Neurturin, Persephin, and Artemin, by a common structural feature called the cysteine-knot. GDNF signals through a multicomponent system of receptors that includes RET and GFRα1-4, to promote dopamine uptake, survival and differentiation of neurons. Recombinant rat GDNF is a non-glycosylated homodimer, containing two 135 amino acid chains, with a total molecular weight of 30.1 kDa.

UniProt: Q07731

Pathways: RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration