TNFSF13 Protein (AA 105-250) (Fc Tag,AVI tag,Biotin)

Catalog No. ABIN6731326

Recombinant TNFSF13 protein expressed in HEK-293 Cells.

Quick Overview for TNFSF13 Protein (AA 105-250) (Fc Tag,AVI tag,Biotin) (ABIN6731326)

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 105-250

Purification tag / Conjugate: This TNFSF13 protein is labelled with Fc Tag,AVI tag,Biotin.

Sequence: AA 105-250

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Alternative Name: APRIL

Background: APRIL(a proliferation-inducing ligand) is also known as Tumor necrosis factor ligand superfamily member 13, TALL-2, TRDL-1, CD256, TNFFSF 13, cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. APRIL is a cytokine of the tumor necrosis factor family associated mainly with hematologic malignancies. The closely related TNF family ligands B cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) serve in the generation and maintenance of mature B-lymphocytes. Both BAFF and APRIL assemble as homotrimers that bind and activate several receptors that they partially share. BAFF-APRIL heteromers of different stoichiometries have distinct receptor-binding properties and activities. In addition, expression of APRIL was regulated by miR-145 in GC cells.

Molecular Weight: 44.6 kDa

NCBI Accession: NP_003799

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response